Michael N. Gould, Ph.D.

M. Gould photo2 Professor of Oncology and Medical Physics

B.S., 1969, Zoology, University of Wisconsin-Madison
M.S., 1973, Radiological Sciences, University of Wisconsin-Madison
Ph.D., 1977, Radiological Sciences, University of Wisconsin-Madison
Postdoctoral research: Argonne National Laboratory

Office: 506B McArdle Laboratory
Telephone: Office - (608) 263-6615; Lab - (608) 263-6026
Email: gould@oncology.wisc.edu

Lab Web Page

Research Interests: Molecular biology of mammary carcinogenesis

Research Description: The focus of Dr. Michael Gould's laboratory is in breast cancer research, including both basic and translational projects. Their major project is in breast cancer genetics. Initially, animal models are being used to look for genetic elements that prevent breast cancer. The human homologs of these elements are then tested as genetic candidates in large breast cancer case-control studies. Candidates showing significant association can serve as risk markers and may also provide a better understanding of the role of modifier genes in breast cancer risk and also provide targets for anti-cancer drug development.

Selected recent publications

Aitman, T. J., Critser, J. K., Cuppen, E., Dominiczak, A., Fernandez-Suarez, X. M., Flint, J., Gauguier, D., Geurts, A. M., Gould, M., Harris, P. C., Holmdahl, R., Hubner, N., Izsvák, Z., Jacob, H. J., Kuramoto, T., Kwitek, A. E., Marrone, A., Mashimo, T., Moreno, C., Mullins, J., Mullins, L., Olsson, T., Pravenec, M., Riley, L., Saar, K., Serikawa, T., Shull, J. D., Szpirer, C., Twigger, S. N., Voigt, B., and Worley, K. Progress and Prospects in Rat Genetics: a Community View. Nat. Genet., 40: 516-522, 2008.

Bauer, M., Eickhoff, J. C., Gould, M. N., Mundhenke, C., Maass, N., and Friedl, A. Neutrophil Gelatinase-associated Lipocalin (NGAL) Is a Predictor of Poor Prognosis in Human Primary Breast Cancer. Breast Cancer Res. Treat., 108: 389-397, 2008.

Woditschka, S., Haag, J. D., Mau, B., Lubet, R. A., and Gould, M. N. Chemopreventive Effects of Celecoxib Are Limited to Hormonally Responsive Mammary Carcinomas in the neu-induced Retroviral Rat Model. Breast Cancer Res., 10:R18, 2008.

Amos-Landgraf, J. M., Kwong, L. N., Kendziorski, C. M., Reichelderfer, M., Torrealba, J., Weichert, J., Haag, J. D., Chen, K.-S., Waller, J. L., Gould, M. N., and Dove, W. F. A Target-selected Apc-mutant Rat Kindred Enhances the Modeling of Familial Human Colon Cancer. Proc. Natl. Acad. Sci. USA, 104: 4036-4041, 2007.

Cotroneo, M. S., Haag, J. D., Zan, Y., Lopez, C. C., Thuwajit, P., Petukhova, G. V., Camerini-Otero, R. D., Gendron-Fitzpatrick, A., Griep, A. E., Murphy, C. J., Dubielzig, R. R., and Gould, M. N. Characterizing a Rat Brca2 Knockout Model. Oncogene, 26: 1626-1635, 2007.

Rose-Hellekant, T. A., Schroeder, M. D., Brockman, J. L., Zhdankin, O., Bolstad, R., Chen, K. S., Gould, M. N., Schuler, L. A., and Sandgren, E. P. Estrogen Receptor-positive Mammary Tumorigenesis in TGFa Transgenic Mice Progresses with Progesterone Receptor Loss. Oncogene, 26: 5238-5246, 2007.

Samuelson, D. J., Hesselson, S. E., Aperavich, B. A., Zan, Y., Haag, J. D., Trentham-Dietz, A., Hampton, J. M., Mau, B., Chen, K.-S., Baynes, C., Khaw, K.-T., Luben, R., Perkins, B., Shah, M., Pharoah, P. D., Dunning, A. M., Easton, D. F., Ponder, B. A., and Gould, M. N. Rat Mcs5a Is a Compound Quantitative Trait Locus with Orthologous Human Loci That Associate with Breast Cancer Risk. Proc. Natl. Acad. Sci. USA, 104: 6299-6304, 2007.

Smits, B. M. G., Cotroneo, M. S., Haag, J. D., and Gould, M. N. Genetically Engineered Rat Models for Breast Cancer. Breast Dis., 28: 53-61, 2007.

Cotroneo, M. S., Merry, G. M., Haag, J. D., Lan, H., Shepel, L. A., and Gould, M. N. The Mcs7 Quantitative Trait Locus Is Associated with an Increased Susceptibility to Mammary Cancer in Congenic Rats and an Allele-specific Imbalance. Oncogene, 25: 5011-5017, 2006.

Woditschka, S., Haag, J. D., Waller, J. L., Monson, D. M., Hitt, A. A., Brose, H. L., Hu, R., Zheng, Y., Watson, P. A., Kim, K., Lindstrom, M. J., Mau, B., Steele, V. E., Lubet, R. A., and Gould, M. N. Neu-Induced Retroviral Rat Mammary Carcinogenesis: A Novel Chemoprevention Model for Both Hormonally Responsive and Nonresponsive Mammary Carcinomas. Cancer Res., 66: 6884-6891, 2006.

Ariazi, J. L., Haag, J. D., and Gould, M. N. Methylguanine Methyltransferase Activity Deficiency in Immature Rat Mammary Epithelial Cells Parallels Increased Carcinogenic Susceptibility. Mol. Carcinog., 44: 193-201, 2005.

Ariazi, J. L., Haag, J. D., Lindstrom, M. J., and Gould, M. N. Mammary Glands of Sexually Immature Rats Are More Susceptible Than Those of Mature Rats to the Carcinogenic, Lethal, and Mutagenic Effects of N-nitroso-N-methylurea. Mol. Carcinog., 43: 155-164, 2005.

Berchtold, C. M., Chen, K.-S., Miyamoto, S., and Gould, M. N. Perillyl Alcohol Inhibits a Calcium-Dependent Constitutive Nuclear Factor-kB Pathway. Cancer Res., 65: 8558-8566, 2005.

Kendziorski, C., Irizarry, R. A., Chen, K.-S., Haag, J. D., and Gould, M. N. On the Utility of Pooling Biological Samples in Microarray Experiments. Proc. Natl. Acad. Sci. USA, 102: 4252-4257, 2005.

Nelson, S. E., Gould, M. N., Hampton, J. M., and Trentham-Dietz, A. A Case-control Study of the HER2 Ile655Val Polymorphism in Relation to Risk of Invasive Breast Cancer. Breast Cancer Res., 7: R357-R364, 2005.

Samuelson, D. J., Aperavich, B. A., Haag, J. D., and Gould, M. N. Fine Mapping Reveals Multiple Loci and a Possible Epistatic Interaction within the Mammary Carcinoma Susceptibility Quantitative Trait Locus, Mcs5. Cancer Res., 65: 9637-9642, 2005.

Chen, K. S., and Gould, M. N. Development of a Universal Gap Repair Vector for Yeast-based Screening of Knockout Rodents. Biotechniques, 37: 383-388, 2004.

Clark, S. S., Zhong, L., Filiault, D., Perman, S., Ren, Z., Gould, M., and Yang, X. Anti-Leukemia Effect of Perillyl Alcohol in Bcr/Abl-Transformed Cells Indirectly Inhibits Signaling through Mek in a Ras- and Raf-Independent Fashion. Clin. Cancer Res., 9: 4494-4504, 2003.

Haag, J. D., Shepel, L. A., Kolman, B. D., Monson, D. M., Benton, M. E., Watts, K. T., Waller, J. L., Lopez-Guajardo, C. C., Samuelson, D. J., and Gould, M. N. Congenic Rats Reveal Three Independent Copenhagen Alleles within the Mcs1 Quantitative Trait Locus That Confer Resistance to Mammary Cancer. Cancer Res., 63: 5808-5812, 2003.

Kendziorski, C. M., Newton, M. A., Lan, H., and Gould, M. N. On Parametric Empirical Bayes Methods for Comparing Multiple Groups Using Replicated Gene Expression Profiles. Statist. Med., 22: 3899-3914, 2003.

McFarlin, D. R., and Gould, M. N. Rat Mammary Carcinogenesis Induced by in Situ Expression of Constitutive Raf Kinase Activity is Prevented by Tethering Raf to the Plasma Membrane. Carcinogenesis, 26: 1149-1153, 2003.

McFarlin, D. R., Lindstrom, M. J., and Gould, M. N. Affinity with Raf is Sufficient for Ras to Efficiently Induce Rat Mammary Carcinomas. Carcinogenesis, 24: 99-105, 2003.

Morgan-Meadows, S., Dubey, S., Gould, M., Tutsch, K., Marnocha, R., Arzoomanin, R., Alberti, D., Binger, K., Feierabend, C., Volkman, J., Ellingen, S., Black, S., Pomplun, M., Wilding, G., and Bailey, H. Phase I Trial of Perillyl Alcohol Administered Four Times Daily Continuously. Cancer Chemother. Pharmacol., 52: 361-366, 2003.

Samuelson, D. J., Haag, J. D., Lan, H., Monson, D. M., Shultz, M. A., Kolman, B. D., and Gould, M. N. Physical Evidence of Mcs5, a QTL Controlling Mammary Carcinoma Susceptibility, in Congenic Rats. Carcinogenesis, 24: 1455-1460, 2003.

Zan, Y., Haag, J. D., Chen, K.-S., Shepel, L. A., Wigington, D., Wang, Y.-R., Hu, R., Lopez-Guajardo, C. C., Brose, H. L., Porter, K. I., Leonard, R. A., Hitt, A. A., Schommer, S. L., Elegbede, A. F., and Gould, M. N. Production of Knockout Rats using ENU Mutagenesis and a Yeast-based Screening Assay. Nat. Biotechnol., 21: 645-651, 2003.

Elegbede, J. A., and Gould, M. N. Monoterpenes Reduced Adducts Formation in Rats Exposed to Aflatoxin B₁. Afr. J. Biotechnol., 1: 46-49, 2002.

Kim, K., Lindstrom, M. J., and Gould, M. N. Regions of H- and K-Ras That Provide Organ Specificity/Potency in Mammary Cancer Induction. Cancer Res., 62: 1241-1245, 2002.

Shi, W., and Gould, M. N. Induction of Cytostasis in Mammary Carcinoma Cells Treated with the Anticancer Agent Perillyl Alcohol. Carcinogenesis, 23: 131-142, 2002.

Shumway, S. D., Berchtold, C. M., Gould, M. N., and Miyamoto, S. Evidence for Unique Calmodulin-Dependent Nuclear Factor-kB Regulation in WEHI-231 B Cells. Mol. Pharmacol., 61: 177-185, 2002.

Thompson, T. A., Haag, J. D., Lindstrom, M. J., Griep, A. E., Lohse, J. K., and Gould, M. N. Decreased Susceptibility to NMU-induced Mammary Carcinogenesis in Transgenic Rats Carrying Multiple Copies of a Rat ras Gene Driven by the Rat Harvey ras Promoter. Oncogene, 21: 2797-2804, 2002.

Watson, P. A., Kim, K., Chen, K.-S., and Gould, M. N. Androgen-dependent Mammary Carcinogenesis in Rats Transgenic for the Neu Proto-Oncogene. Cancer Cell, 2: 67-79, 2002.

Lan, H., Kendziorski, C. M., Haag, J. D., Shepel, L. A., Newton, M. A., and Gould, M. N. Genetic Loci Controlling Breast Cancer Susceptibility in the Wistar-Kyoto Rat. Genetics, 157: 331-339, 2001.

Ripple, G. H., Gould, M. N., Arzoomanian, R. Z., Alberti, D., Feierabend, C., Simon, K., Binger, K., Tutsch, K. D., Pomplun, M., Wahamaki, A., Marnocha, R., Wilding, G., and Bailey, H. H. Phase I Clinical and Pharmacokinetic Study of Perillyl Alcohol Administered Four Times a Day. Clin. Cancer Res., 6: 390-396, 2000.

Thompson, T. A., and Gould, M. N. Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectors. In: M. M. Ip and B. B. Asch (Eds.), Methods in Mammary Gland Biology and Breast Cancer Research, Chapter 22, pp. 245-257. New York: Kluwer Academic/Plenum Publ., 2000.

Thompson, T. A., Haag, J. D., and Gould, M. N. ras Gene Mutations Are Absent in NMU-induced Mammary Carcinomas from Aging Rats. Carcinogenesis, 21: 1917-1922, 2000.

Ariazi, E. A., Satomi, Y., Ellis, M. J., Haag, J. D., Shi, W., Sattler, C. A., and Gould, M. N. Activation of the Transforming Growth Factor b Signaling Pathway and Induction of Cytostasis and Apoptosis in Mammary Carcinomas Treated with the Anticancer Agent Perillyl Alcohol. Cancer Res., 59: 1917-1928, 1999.

Benton, M. E., Chen, K.-S., Haag, J. D., Sattler, C. A., and Gould, M. N. Precocious Differentiation of the Virgin Wistar-Kyoto Rat Mammary Gland. Endocrinology, 140: 2659-2671, 1999.

Friedl, A., Stoesz, S. P., Buckley, P., and Gould, M. N. Neutrophil Gelatinase-associated Lipocalin in Normal and Neoplastic Human Tissues. Cell Type-specific Pattern of Expression. Histochem. J., 31: 433-441, 1999.

Haag, J. D., Brasic, G. M., Shepel, L. A., Newton, M. A., Grubbs, C. J., Lubet, R. A., Kelloff, G. J., and Gould, M. N. A Comparative Analysis of Allelic Imbalance Events in Chemically Induced Rat Mammary, Colon, and Bladder Tumors. Mol. Carcinog., 24: 47-56, 1999.

Lan, H., Shepel, L. A., Haag, J. D., and Gould, M. N. Linkage Mapping of Rat Chromosome 5 Markers Generated from Chromosome-specific Libraries. Mamm. Genome, 10: 687-691, 1999.

Satomi, Y., Miyamoto, S., and Gould, M. N. Induction of AP-1 Activity by Perillyl Alcohol in Breast Cancer Cells. Carcinogenesis, 20: 1957-1961, 1999.

Shepel, L. A., and Gould, M. N. The Genetic Components of Susceptibility to Breast Cancer in the Rat. In: H. Hiai and O. Hino (Eds.), Animal Models of Cancer Predisposition Syndromes, Prog. Exp. Tumor Res., Vol. 35, pp. 158-169. Basel: Karger, 1999.

Hill, C. K., Nelms, B. E., MacKay, J. F., Pearson, D. W., Kennan, W. S., Mackie, T. R., DeLuca, P. M., Jr., Lindstrom, M. J., and Gould, M. N. Synchrotron-produced Ultrasoft X Rays: Equivalent Cell Survival at the Isoattenuating Energies 273 eV and 860 eV. Radiat. Res., 150: 513-520, 1998.

Kamiya, K., Gould, M. N., and Clifton, K. H. Quantitative Studies of Ductal Versus Alveolar Differentiation from Rat Mammary Clonogens. Proc. Soc. Exp. Biol. Med., 219: 217-225, 1998.

MacKay, J. F., Pearson, D. W., Nelms, B. E., DeLuca, P. M., Gould, M. N., and Lagally, M. G. A Double Mirror W/C Multilayer Monochromator for Radiation Biology Applications. Medical Physics, 25: 773-779, 1998.

Nelms, B. E., Mackie, R. T., Mackay, J. F., Hill, C. K., DeLuca, P. M., Jr., Lindstrom, M. J., Deasy, J., and Gould, M. N. A Comparison of Cytotoxicity After Whole- or Partial-cell Irradiation with Synchrotron-produced Ultrasoft X-rays. Radiat. Res., 150: 521-527, 1998.

Newton, M. A., Gould, M. N., Reznikoff, C. A., and Haag, J. D. On the Statistical Analysis of Allelic-loss Data. Statist. Med., 17: 1425-1445, 1998.

Ren, Z., and Gould, M. N. Modulation of Small G Protein Isoprenylation by Anticancer Monoterpenes in in Situ Mammary Gland Epithelial Cells. Carcinogenesis, 19: 827-832, 1998.

Ripple, G. H, Gould, M. N., Stewart, J. A., Tutsch, K. D., Arzoomanian, R. Z., Alberti, D., Feierabend, C., Pomplun, M., Wilding, G., and Bailey, H. H. Phase I Clinical Trial of Perillyl Alcohol Administered Daily. Clin. Cancer Res., 4: 1159-1164, 1998.

Shepel, L. A., Fleming, R. E., Haag, J. D., Brasic, G. M., Gheen, M. E., Simon, J. S., and Gould, M. N. Genetic Linkage Mapping of the Rat Ceruloplasmin (Cp) Gene and 11 Novel DNA Markers on Chromosome 2. Cytogenet. Cell Genet., 79: 176-178, 1998.

Shepel, L. A., Lan, H., Brasic, G. M., Gheen, M. E., Haag, J. D., and Gould, M. N. Linkage Mapping of Rat Microsatellite Markers from Chromosome-specific Libraries. Mammalian Genome, 9: 622-628, 1998.

Shepel, L. A., Lan, H., Haag, J. D., Brasic, G. M., Gheen, M. E., Simon, J. S., Hoff, P., Newton, M. A., and Gould, M. N. Genetic Identification of Multiple Loci that Control Breast Cancer Susceptibility in the Rat. Genetics, 149: 289-299, 1998.

Stoesz, S. P., Friedl, A., Haag, J. D., Lindstrom, M. J., Clark, G. M., and Gould, M. N. Heterogeneous Expression of the Lipocalin N-gal in Primary Breast Cancers. Int. J. Cancer, 79: 565-572, 1998.

Thompson, T. A., Kim, K., and Gould, M. N. Harvey ras Results in a Higher Frequency of Mammary Carcinomas than Kirsten ras after Direct Retroviral Transfer into the Rat Mammary Gland. Cancer Res., 58: 5097-5104, 1998.